You have been allocated a cDNA accession number from the NCBI database (see table below). Your task is to design an experimental strategy that will allow you to clone the cDNA, express the protein, and finally confirm that it is the correct protein using tryptic digestion and MASCOT analysis.
Completed cover page (excluding Primers for PCR & cloning)
pEGFP-N1 responses (on cover page, marks deducted for inaccuracies at each point)
pH6HTN His6HaloTagT7 responses (on cover page, marks deducted for inaccuracies at each point))
150 word description of the function of your protein (marks deducted for poor use of language including spelling, grammar, punctuation and scientific terminology)
150 word explanation of your cloning strategy (marks deducted for poor use of language including spelling, grammar, punctuation and scientific terminology)
coding region of your allocated cDNA sequence (do not include non coding regions as marks will be deducted for this)
restriction map of your coding region (include a list of non-cutting enzymes)
Plasmid maps for both plasmids (marks will be deducted for poor quality images & presentation)
translated sequence of your coding region (aligned with the cDNA)
translated sequence of your full length fusion protein (aligned with the cDNA sequence)
theoretical tryptic digest of your untagged protein (marks deducted for errors)
theoretical tryptic digest of your tagged/fused protein (marks deducted for errors)
Results from MASCOT analysis of tryptic digest (marks deducted for poor presentation and errors)

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